| Date | Home | Away | Result |
|---|---|---|---|
| 12/3/24 12:00 PM | Real Avila | Real Valladolid | 2-4a.e.t |
| 12/3/24 1:00 PM | Yeclano Deportivo | FC Elche | 0-1 |
| 12/3/24 2:00 PM | Salamanca CF UDS | Celta Vigo | 0-7 |
| 12/3/24 2:00 PM | UD Barbastro | Espanyol Barcelona | 2-0 |
| 12/3/24 2:00 PM | Real Saragossa | FC Granada | 6-7a.PSO |
| 12/3/24 2:15 PM | CE Europa | UD Las Palmas | 1-2 |
| 12/4/24 12:00 PM | Unionistas de Salamanca | Rayo Vallecano | 2-3 |
| 12/4/24 12:00 PM | Gimnastic de Tarragona | SD Huesca | 0-1 |
| 12/4/24 12:00 PM | UE Sant Andreu | Real Betis Sevilla | 1-3 |
| 12/4/24 1:00 PM | CF Ourense | Deportivo La Coruna | 1-0 |
| 12/4/24 1:00 PM | Cultural y Deportiva Leonesa | UD Almeria | 1-2 |
| 12/4/24 1:00 PM | FC Cadiz | CD Eldense | 0-1 |
| 12/4/24 1:30 PM | Racing Santander | Sporting Gijon | 1-0 |
| 12/4/24 2:00 PM | UD Logrones | FC Girona | 4-3a.PSO |
| 12/4/24 2:00 PM | CF Pontevedra | FC Villarreal | 1-0 |
| 12/4/24 2:00 PM | SD Ejea | FC Valencia | 1-3 |
| 12/4/24 2:00 PM | Zamora CF | CD Teneriffa | 1-3a.PSO |
| 12/4/24 2:00 PM | CD Estepona | CD Leganes | 6-7a.PSO |
| 12/5/24 12:00 PM | CP Cacereno | Atletico Madrid | 1-3 |
| 12/5/24 12:00 PM | AD Ceuta FC | CA Osasuna | 2-3 |
| 12/5/24 12:00 PM | Orihuela CF | FC Getafe | 0-3a.PSO |
| 12/5/24 1:00 PM | Andorra CF | FC Cartagena | 0-1 |
| 12/5/24 1:00 PM | Marbella FC | Burgos CF | 1-0 |
| 12/5/24 1:00 PM | SD Ponferradina | CD Castellon | 5-4a.PSO |
| 12/5/24 1:00 PM | Barakaldo CF | Racing de Ferrol | 1-2 |
| 12/5/24 2:00 PM | Ub Conquense | Real Sociedad | 0-1a.e.t |
| 12/5/24 2:00 PM | Olot | FC Sevilla | 1-3 |
| 12/5/24 2:00 PM | Deportiva Minera | Deportivo Alaves | 6-4a.PSO |