| 1/5/21 10:00 AM | UD Ibiza Eivissa | Celta Vigo | 5-2 |
| 1/5/21 10:00 AM | Cordoba CF | FC Getafe | 1-0 |
| 1/5/21 12:00 PM | Zamora CF | FC Villarreal | 1-4 |
| 1/5/21 12:00 PM | Linares Deportivo | FC Sevilla | 0-2 |
| 1/5/21 2:00 PM | AD Alcorcon | Real Saragossa | 2-1 |
| 1/5/21 2:15 PM | Marbella FC | Real Valladolid | 2-3a.e.t |
| 1/6/21 5:00 AM | CF La Nucia | FC Elche | 0-1 |
| 1/6/21 5:00 AM | Club Portugalete | UD Levante | 1-2 |
| 1/6/21 5:00 AM | CD Numancia | UD Almeria | 1-2 |
| 1/6/21 6:00 AM | UD Socuellamos | CD Leganes | 0-2 |
| 1/6/21 10:00 AM | CD Artistico Navalcarnero | UD Las Palmas | 1-0 |
| 1/6/21 10:00 AM | Club Haro Deportivo | Rayo Vallecano | 1-3 |
| 1/6/21 10:00 AM | Cultural y Deportiva Leonesa | FC Granada | 1-2a.e.t |
| 1/6/21 10:00 AM | Union Mutilvera | Real Betis Sevilla | 1-3 |
| 1/6/21 11:00 AM | UE Cornella | Atletico Madrid | 1-0 |
| 1/6/21 12:00 PM | Deportivo La Coruna | Deportivo Alaves | 0-1 |
| 1/6/21 12:00 PM | Olot | CA Osasuna | 0-3 |
| 1/6/21 12:00 PM | Burgos CF | Espanyol Barcelona | 0-2 |
| 1/6/21 1:00 PM | CD Castellon | CD Teneriffa | 0-2 |
| 1/6/21 2:00 PM | CF Fuenlabrada | RCD Mallorca | 9-8a.PSO |
| 1/6/21 2:00 PM | FC Malaga | Real Oviedo | 1-0a.e.t |
| 1/7/21 12:00 PM | CF Pontevedra | FC Cadiz | 4-5a.PSO |
| 1/7/21 12:00 PM | SD Amorebieta | Sporting Gijon | 0-1 |
| 1/7/21 12:00 PM | Deportiva Santa Eulalia del Rio | CD Sabadell | 2-0a.PSO |
| 1/7/21 12:00 PM | FC Girona | CD Lugo | 2-1a.e.t |
| 1/7/21 12:00 PM | Yeclano Deportivo | FC Valencia | 1-4 |
| 1/7/21 2:00 PM | Las Rozas CF | SD Eibar | 3-4a.e.t |
| 1/7/21 2:00 PM | CD Alcoyano | SD Huesca | 2-1 |
