| Date | Home | Away | Result |
|---|---|---|---|
| 5/23/21 8:30 AM | Hamburger SV | Eintracht Braunschweig | 4-0 |
| 5/23/21 8:30 AM | Hannover 96 | 1. FC Nürnberg | 1-2 |
| 5/23/21 8:30 AM | FC Würzburger Kickers | SC Paderborn 07 | 1-1 |
| 5/23/21 8:30 AM | Erzgebirge Aue | VfL Osnabrück | 2-1 |
| 5/23/21 8:30 AM | VfL Bochum | SV Sandhausen | 3-1 |
| 5/23/21 8:30 AM | Holstein Kiel | SV Darmstadt 98 | 2-3 |
| 5/23/21 8:30 AM | Jahn Regensburg | FC St. Pauli | 3-0 |
| 5/23/21 8:30 AM | SpVgg Greuther Fürth | Fortuna Düsseldorf | 3-2 |
| 5/23/21 8:30 AM | 1. FC Heidenheim 1846 | Karlsruher SC | 1-2 |