| Date | Home | Away | Result |
|---|---|---|---|
| 5/15/22 8:30 AM | Werder Bremen | Jahn Regensburg | 2-0 |
| 5/15/22 8:30 AM | SV Darmstadt 98 | SC Paderborn 07 | 3-0 |
| 5/15/22 8:30 AM | 1. FC Heidenheim 1846 | Karlsruher SC | 2-0 |
| 5/15/22 8:30 AM | FC St. Pauli | Fortuna Düsseldorf | 2-0 |
| 5/15/22 8:30 AM | 1. FC Nürnberg | FC Schalke 04 | 1-2 |
| 5/15/22 8:30 AM | Hannover 96 | FC Ingolstadt | 3-2 |
| 5/15/22 8:30 AM | SV Sandhausen | Holstein Kiel | 3-1 |
| 5/15/22 8:30 AM | Dynamo Dresden | Erzgebirge Aue | 0-1 |
| 5/15/22 8:30 AM | FC Hansa Rostock | Hamburger SV | 2-3 |