| Date | Home | Away | Result |
|---|---|---|---|
| 5/10/21 7:00 AM | SPAL Ferrara | US Cremonese | 1-0 |
| 5/10/21 7:00 AM | Vicenza Calcio | Reggio Audace FC | 2-1 |
| 5/10/21 7:00 AM | AC Monza | Brescia Calcio | 0-2 |
| 5/10/21 7:00 AM | FC Empoli | US Lecce | 2-1 |
| 5/10/21 7:00 AM | AC Chievo Verona | Ascoli Calcio | 3-0 |
| 5/10/21 7:00 AM | Pescara Calcio | US Salernitana | 0-3 |
| 5/10/21 7:00 AM | AS Cittadella | FC Venice | 1-1 |
| 5/10/21 7:00 AM | Pordenone | Cosenza Calcio | 2-0 |
| 5/10/21 7:00 AM | AC Pisa | Virtus Entella | 3-2 |
| 5/10/21 7:00 AM | Reggina 1914 | Frosinone Calcio | 0-4 |